Researchers at Carnegie Mellon University have announced a technique that could make control of neurons using light an easier process. The team was led by associate professor of biomedical engineering and materials science and engineering Tzahi Cohen-Karni. Cohen-karni’s team used a nanowire template to synthesize 3D fuzzy graphene, creating a material that could more easily photothermally stimulate cells, dubbed NW-templated three-dimensional (3D) fuzzy graphene (NT-3DFG). This material allows researchers to optically stimulated neurons without using genetic modification. NT-3DFG also uses far less energy than other materials, minimizing cellular stress.
Graphene is abundant, cheap, and biocompatible. Cohen-Karni's lab has been working with graphene for several years, developing a technique of synthesizing the material in 3D topologies that he's labeled "fuzzy" graphene. By growing two-dimensional (2D) graphene flakes out-of-plane on a silicon nanowire structure, they're able to create a 3D structure with broadband optical absorption and unparalleled photothermal efficiency.
These properties make it ideal for cellular electrophysiology modulation using light through the optocapacitive effect. The optocapacitive effect alters the cell membrane capacitance due to rapidly applied light pulses. NT-3DFG can be readily made in suspension, allowing the study of cell signaling within and between both 2D cell systems and 3D, like human cell-based organoids.
Systems like these are not only crucial to understanding how cells signal and interact with each other, but also hold great potential for the development of new, therapeutic interventions. Exploration into these opportunities, however, has been limited by the risk of cellular stress that existing optical remote-control technologies present. The use of NT-3DFG eliminates this risk by using significantly less energy, on a scale of 1-2 orders of magnitude less. Its biocompatible surface is easy to modify chemically, making it versatile for use with different cell types and environments. Using NT-3DFG, photothermal stimulation treatments could be developed for motor recruitment to induce muscle activation or could direct tissue development in an organoid system.
"This is an outstanding collaborative work of experts from multiple fields, including neuroscience through Pitt and UChicago, and photonics and materials science through UNC and CMU," said Cohen-Karni. "The developed technology will allow us to interact with either engineered tissues or with nerve or muscle tissue in vivo. This will allow us to control and affect tissue functionality using light remotely with high precision and low needed energies."
Additional contributions to the project were made by Maysam Chamanzar, assistant professor of electrical and computer engineering. His team's core expertise in photonics and neurotechnologies assisted in developing the much-needed tools to allow both the characterization of the unique hybrid-nanomaterials, and in stimulating the cells while optically recording their activity.
"The broadband absorption of these 3D nanomaterials enabled us to use light at wavelengths that can penetrate deep into the tissue to remotely excite nerve cells. This method can be used in a whole gamut of applications, from designing non-invasive therapeutics to basic scientific studies," said Chamanzar.
The team's findings are significant both for our understanding of cell interactions and the development of therapies that harness the potential of the human body's own cells. Nanostructures created using NT-3DFG may have a major impact on the future of human biology and medicine.
Reference: Rastogi, S. K., Garg, R., Scopelliti, M. G., Pinto, B. I., Hartung, J. E., Kim, S., Murphey, C. G. E., Johnson, N., Roman, D. S., Bezanilla, F., Cahoon, J. F., Gold, M. S., Chamanzar, M., & Cohen-Karni, T. (2020). Remote nongenetic optical modulation of neuronal activity using fuzzy graphene. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1919921117
This article has been republished from materials provided by Carnegie Mellon University. Note: material may have been edited for length and content. For further information, please contact the cited source.
"light" - Google News
June 03, 2020 at 05:27PM
https://ift.tt/304kjCY
Light-based Technique Is a Remote Control for Neurons - Technology Networks
"light" - Google News
https://ift.tt/2Wm8QLw
https://ift.tt/2Stbv5k
Bagikan Berita Ini
0 Response to "Light-based Technique Is a Remote Control for Neurons - Technology Networks"
Post a Comment